pcr-direct sequence method Search Results


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Oxford Nanopore direct rna and pcr-cdna sequencing method
Comparative analysis of <t>aligned</t> <t>sequencing</t> reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct <t>RNA</t> sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript
Direct Rna And Pcr Cdna Sequencing Method, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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direct rna and pcr-cdna sequencing method - by Bioz Stars, 2026-07
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Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

Journal: BMC Genomics

Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

doi: 10.1186/s12864-022-08386-y

Figure Lengend Snippet: Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

Techniques: Sequencing, Derivative Assay, RNA Sequencing, Nanopore Sequencing, Generated

Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

Journal: BMC Genomics

Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

doi: 10.1186/s12864-022-08386-y

Figure Lengend Snippet: Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

Techniques: Sequencing, Modification